Part:BBa_K3861016

AIDA autotransporter

Sequence of the AIDA-I autotransporter derived from pRAIDA2.¹ Must be used together with the signal sequence (BBa_K3861013) to allow display of a protein of interest (POI) on the bacterial surface. The gene encoding for the POI is to be inserted downstream of the signal sequence and upstream of the autotransporter. We combined the parts to create a construct with the potential ability to display the recombinant lectin rBC2LCN (BBa_K3861001) on the surface of bacterial minicells in order to enable active targeting of MCF7 breast cancer cells by attaching to glycans on the cancer cell surface (BBa_K3861018).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 773
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Yu, H. et al. Minicells from Highly Genome Reduced Escherichia coli: Cytoplasmic and Surface Expression of Recombinant Proteins and Incorporation in the Minicells, ACS Synthetic Biology, (2021).